Dynamic DNA cytosine methylation in the Populus trichocarpa genome: tissue-level variation and relationship to gene expression.
Identifieur interne : 002B21 ( Main/Exploration ); précédent : 002B20; suivant : 002B22Dynamic DNA cytosine methylation in the Populus trichocarpa genome: tissue-level variation and relationship to gene expression.
Auteurs : Kelly J. Vining [États-Unis] ; Kyle R. Pomraning ; Larry J. Wilhelm ; Henry D. Priest ; Matteo Pellegrini ; Todd C. Mockler ; Michael Freitag ; Steven H. StraussSource :
- BMC genomics [ 1471-2164 ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Cytosine.
- genetics : Chromosomes, Plant, Populus.
- metabolism : Populus.
- DNA Methylation, Epigenesis, Genetic, Gene Expression Regulation, Plant, Promoter Regions, Genetic, Sequence Analysis.
Abstract
BACKGROUND
DNA cytosine methylation is an epigenetic modification that has been implicated in many biological processes. However, large-scale epigenomic studies have been applied to very few plant species, and variability in methylation among specialized tissues and its relationship to gene expression is poorly understood.
RESULTS
We surveyed DNA methylation from seven distinct tissue types (vegetative bud, male inflorescence [catkin], female catkin, leaf, root, xylem, phloem) in the reference tree species black cottonwood (Populus trichocarpa). Using 5-methyl-cytosine DNA immunoprecipitation followed by Illumina sequencing (MeDIP-seq), we mapped a total of 129,360,151 36- or 32-mer reads to the P. trichocarpa reference genome. We validated MeDIP-seq results by bisulfite sequencing, and compared methylation and gene expression using published microarray data. Qualitative DNA methylation differences among tissues were obvious on a chromosome scale. Methylated genes had lower expression than unmethylated genes, but genes with methylation in transcribed regions ("gene body methylation") had even lower expression than genes with promoter methylation. Promoter methylation was more frequent than gene body methylation in all tissues except male catkins. Male catkins differed in demethylation of particular transposable element categories, in level of gene body methylation, and in expression range of genes with methylated transcribed regions. Tissue-specific gene expression patterns were correlated with both gene body and promoter methylation.
CONCLUSIONS
We found striking differences among tissues in methylation, which were apparent at the chromosomal scale and when genes and transposable elements were examined. In contrast to other studies in plants, gene body methylation had a more repressive effect on transcription than promoter methylation.
DOI: 10.1186/1471-2164-13-27
PubMed: 22251412
PubMed Central: PMC3298464
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Dynamic DNA cytosine methylation in the Populus trichocarpa genome: tissue-level variation and relationship to gene expression.</title><author><name sortKey="Vining, Kelly J" sort="Vining, Kelly J" uniqKey="Vining K" first="Kelly J" last="Vining">Kelly J. Vining</name><affiliation wicri:level="1"><nlm:affiliation>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, 97331, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, 97331</wicri:regionArea><wicri:noRegion>97331</wicri:noRegion></affiliation></author><author><name sortKey="Pomraning, Kyle R" sort="Pomraning, Kyle R" uniqKey="Pomraning K" first="Kyle R" last="Pomraning">Kyle R. Pomraning</name></author><author><name sortKey="Wilhelm, Larry J" sort="Wilhelm, Larry J" uniqKey="Wilhelm L" first="Larry J" last="Wilhelm">Larry J. Wilhelm</name></author><author><name sortKey="Priest, Henry D" sort="Priest, Henry D" uniqKey="Priest H" first="Henry D" last="Priest">Henry D. Priest</name></author><author><name sortKey="Pellegrini, Matteo" sort="Pellegrini, Matteo" uniqKey="Pellegrini M" first="Matteo" last="Pellegrini">Matteo Pellegrini</name></author><author><name sortKey="Mockler, Todd C" sort="Mockler, Todd C" uniqKey="Mockler T" first="Todd C" last="Mockler">Todd C. Mockler</name></author><author><name sortKey="Freitag, Michael" sort="Freitag, Michael" uniqKey="Freitag M" first="Michael" last="Freitag">Michael Freitag</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22251412</idno><idno type="pmid">22251412</idno><idno type="doi">10.1186/1471-2164-13-27</idno><idno type="pmc">PMC3298464</idno><idno type="wicri:Area/Main/Corpus">002B66</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002B66</idno><idno type="wicri:Area/Main/Curation">002B66</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002B66</idno><idno type="wicri:Area/Main/Exploration">002B66</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Dynamic DNA cytosine methylation in the Populus trichocarpa genome: tissue-level variation and relationship to gene expression.</title><author><name sortKey="Vining, Kelly J" sort="Vining, Kelly J" uniqKey="Vining K" first="Kelly J" last="Vining">Kelly J. Vining</name><affiliation wicri:level="1"><nlm:affiliation>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, 97331, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, 97331</wicri:regionArea><wicri:noRegion>97331</wicri:noRegion></affiliation></author><author><name sortKey="Pomraning, Kyle R" sort="Pomraning, Kyle R" uniqKey="Pomraning K" first="Kyle R" last="Pomraning">Kyle R. Pomraning</name></author><author><name sortKey="Wilhelm, Larry J" sort="Wilhelm, Larry J" uniqKey="Wilhelm L" first="Larry J" last="Wilhelm">Larry J. Wilhelm</name></author><author><name sortKey="Priest, Henry D" sort="Priest, Henry D" uniqKey="Priest H" first="Henry D" last="Priest">Henry D. Priest</name></author><author><name sortKey="Pellegrini, Matteo" sort="Pellegrini, Matteo" uniqKey="Pellegrini M" first="Matteo" last="Pellegrini">Matteo Pellegrini</name></author><author><name sortKey="Mockler, Todd C" sort="Mockler, Todd C" uniqKey="Mockler T" first="Todd C" last="Mockler">Todd C. Mockler</name></author><author><name sortKey="Freitag, Michael" sort="Freitag, Michael" uniqKey="Freitag M" first="Michael" last="Freitag">Michael Freitag</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author></analytic><series><title level="j">BMC genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromosomes, Plant (genetics)</term><term>Cytosine (metabolism)</term><term>DNA Methylation (MeSH)</term><term>Epigenesis, Genetic (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Sequence Analysis (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence (MeSH)</term><term>Chromosomes de plante (génétique)</term><term>Cytosine (métabolisme)</term><term>Méthylation de l'ADN (MeSH)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Épigenèse génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytosine</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chromosomes, Plant</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chromosomes de plante</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cytosine</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>DNA Methylation</term><term>Epigenesis, Genetic</term><term>Gene Expression Regulation, Plant</term><term>Promoter Regions, Genetic</term><term>Sequence Analysis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence</term><term>Méthylation de l'ADN</term><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes végétaux</term><term>Épigenèse génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>DNA cytosine methylation is an epigenetic modification that has been implicated in many biological processes. However, large-scale epigenomic studies have been applied to very few plant species, and variability in methylation among specialized tissues and its relationship to gene expression is poorly understood.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>We surveyed DNA methylation from seven distinct tissue types (vegetative bud, male inflorescence [catkin], female catkin, leaf, root, xylem, phloem) in the reference tree species black cottonwood (Populus trichocarpa). Using 5-methyl-cytosine DNA immunoprecipitation followed by Illumina sequencing (MeDIP-seq), we mapped a total of 129,360,151 36- or 32-mer reads to the P. trichocarpa reference genome. We validated MeDIP-seq results by bisulfite sequencing, and compared methylation and gene expression using published microarray data. Qualitative DNA methylation differences among tissues were obvious on a chromosome scale. Methylated genes had lower expression than unmethylated genes, but genes with methylation in transcribed regions ("gene body methylation") had even lower expression than genes with promoter methylation. Promoter methylation was more frequent than gene body methylation in all tissues except male catkins. Male catkins differed in demethylation of particular transposable element categories, in level of gene body methylation, and in expression range of genes with methylated transcribed regions. Tissue-specific gene expression patterns were correlated with both gene body and promoter methylation.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>We found striking differences among tissues in methylation, which were apparent at the chromosomal scale and when genes and transposable elements were examined. In contrast to other studies in plants, gene body methylation had a more repressive effect on transcription than promoter methylation.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22251412</PMID><DateCompleted><Year>2012</Year><Month>05</Month><Day>23</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2164</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><PubDate><Year>2012</Year><Month>Jan</Month><Day>17</Day></PubDate></JournalIssue><Title>BMC genomics</Title><ISOAbbreviation>BMC Genomics</ISOAbbreviation></Journal><ArticleTitle>Dynamic DNA cytosine methylation in the Populus trichocarpa genome: tissue-level variation and relationship to gene expression.</ArticleTitle><Pagination><MedlinePgn>27</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/1471-2164-13-27</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">DNA cytosine methylation is an epigenetic modification that has been implicated in many biological processes. However, large-scale epigenomic studies have been applied to very few plant species, and variability in methylation among specialized tissues and its relationship to gene expression is poorly understood.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">We surveyed DNA methylation from seven distinct tissue types (vegetative bud, male inflorescence [catkin], female catkin, leaf, root, xylem, phloem) in the reference tree species black cottonwood (Populus trichocarpa). Using 5-methyl-cytosine DNA immunoprecipitation followed by Illumina sequencing (MeDIP-seq), we mapped a total of 129,360,151 36- or 32-mer reads to the P. trichocarpa reference genome. We validated MeDIP-seq results by bisulfite sequencing, and compared methylation and gene expression using published microarray data. Qualitative DNA methylation differences among tissues were obvious on a chromosome scale. Methylated genes had lower expression than unmethylated genes, but genes with methylation in transcribed regions ("gene body methylation") had even lower expression than genes with promoter methylation. Promoter methylation was more frequent than gene body methylation in all tissues except male catkins. Male catkins differed in demethylation of particular transposable element categories, in level of gene body methylation, and in expression range of genes with methylated transcribed regions. Tissue-specific gene expression patterns were correlated with both gene body and promoter methylation.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">We found striking differences among tissues in methylation, which were apparent at the chromosomal scale and when genes and transposable elements were examined. In contrast to other studies in plants, gene body methylation had a more repressive effect on transcription than promoter methylation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vining</LastName><ForeName>Kelly J</ForeName><Initials>KJ</Initials><AffiliationInfo><Affiliation>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, 97331, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pomraning</LastName><ForeName>Kyle R</ForeName><Initials>KR</Initials></Author><Author ValidYN="Y"><LastName>Wilhelm</LastName><ForeName>Larry J</ForeName><Initials>LJ</Initials></Author><Author ValidYN="Y"><LastName>Priest</LastName><ForeName>Henry D</ForeName><Initials>HD</Initials></Author><Author ValidYN="Y"><LastName>Pellegrini</LastName><ForeName>Matteo</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Mockler</LastName><ForeName>Todd C</ForeName><Initials>TC</Initials></Author><Author ValidYN="Y"><LastName>Freitag</LastName><ForeName>Michael</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Strauss</LastName><ForeName>Steven H</ForeName><Initials>SH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>01</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Genomics</MedlineTA><NlmUniqueID>100965258</NlmUniqueID><ISSNLinking>1471-2164</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>8J337D1HZY</RegistryNumber><NameOfSubstance UI="D003596">Cytosine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D032461" MajorTopicYN="N">Chromosomes, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003596" MajorTopicYN="N">Cytosine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019175" MajorTopicYN="Y">DNA Methylation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044127" MajorTopicYN="N">Epigenesis, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017421" MajorTopicYN="N">Sequence Analysis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>09</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>01</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>1</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>1</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>5</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22251412</ArticleId><ArticleId IdType="pii">1471-2164-13-27</ArticleId><ArticleId IdType="doi">10.1186/1471-2164-13-27</ArticleId><ArticleId IdType="pmc">PMC3298464</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Annu Rev Plant Biol. 2009;60:43-66</Citation><ArticleIdList><ArticleId IdType="pubmed">19007329</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Biol. 2007 Jul;5(7):e174</Citation><ArticleIdList><ArticleId IdType="pubmed">17579518</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2009 Apr;21(4):1053-69</Citation><ArticleIdList><ArticleId IdType="pubmed">19376930</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2009 Jun 12;324(5933):1447-51</Citation><ArticleIdList><ArticleId IdType="pubmed">19520961</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2009 Jun 12;324(5933):1451-4</Citation><ArticleIdList><ArticleId IdType="pubmed">19520962</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2009;10(6):R62</Citation><ArticleIdList><ArticleId IdType="pubmed">19508735</ArticleId></ArticleIdList></Reference><Reference><Citation>J Plant Physiol. 2009 Sep 1;166(13):1360-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19376609</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2009 Sep 17;461(7262):427-30</Citation><ArticleIdList><ArticleId IdType="pubmed">19734882</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2009;10(8):232</Citation><ArticleIdList><ArticleId IdType="pubmed">19725931</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Genet. 2009 Nov;25(11):511-7</Citation><ArticleIdList><ArticleId IdType="pubmed">19850370</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2010 Jan;20(1):45-58</Citation><ArticleIdList><ArticleId IdType="pubmed">19858364</ArticleId></ArticleIdList></Reference><Reference><Citation>J Plant Physiol. 2010 Mar 15;167(5):351-7</Citation><ArticleIdList><ArticleId IdType="pubmed">19931210</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2010 Jan;22(1):17-33</Citation><ArticleIdList><ArticleId IdType="pubmed">20086188</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2010;5(3):e9514</Citation><ArticleIdList><ArticleId IdType="pubmed">20209086</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2010;11:150</Citation><ArticleIdList><ArticleId IdType="pubmed">20199690</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2010 May 11;107(19):8689-94</Citation><ArticleIdList><ArticleId IdType="pubmed">20395551</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2010 May 14;328(5980):916-9</Citation><ArticleIdList><ArticleId IdType="pubmed">20395474</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant. 2010 May;3(3):594-602</Citation><ArticleIdList><ArticleId IdType="pubmed">20410255</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18729-34</Citation><ArticleIdList><ArticleId IdType="pubmed">20937895</ArticleId></ArticleIdList></Reference><Reference><Citation>Sex Plant Reprod. 2010 Dec;23(4):315-23</Citation><ArticleIdList><ArticleId IdType="pubmed">20552230</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2010;11(10):R106</Citation><ArticleIdList><ArticleId IdType="pubmed">20979621</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Signal Behav. 2010 Aug;5(8):995-8</Citation><ArticleIdList><ArticleId IdType="pubmed">20724818</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Genet. 2010 Mar;11(3):191-203</Citation><ArticleIdList><ArticleId IdType="pubmed">20125086</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12521-6</Citation><ArticleIdList><ArticleId IdType="pubmed">21746919</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2012 Jan;22(1):95-105</Citation><ArticleIdList><ArticleId IdType="pubmed">21974993</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2009 Mar;19(3):427-37</Citation><ArticleIdList><ArticleId IdType="pubmed">19092133</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Biochem. 2005;74:481-514</Citation><ArticleIdList><ArticleId IdType="pubmed">15952895</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Genet. 2005 Aug;37(8):853-62</Citation><ArticleIdList><ArticleId IdType="pubmed">16007088</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell. 2000 Oct;6(4):791-802</Citation><ArticleIdList><ArticleId IdType="pubmed">11090618</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2002 Jul 12;110(1):33-42</Citation><ArticleIdList><ArticleId IdType="pubmed">12150995</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2002 Aug;215(4):672-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12172851</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2002 Aug;22(11):813-6</Citation><ArticleIdList><ArticleId IdType="pubmed">12184986</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2003 Mar;163(3):1109-22</Citation><ArticleIdList><ArticleId IdType="pubmed">12663548</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2003 Apr 24;422(6934):893-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12712205</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Biol. 2003 Dec 16;13(24):2212-7</Citation><ArticleIdList><ArticleId IdType="pubmed">14680640</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2004 Apr;24(4):401-7</Citation><ArticleIdList><ArticleId IdType="pubmed">14757579</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2004 Mar;22(8):594-8</Citation><ArticleIdList><ArticleId IdType="pubmed">14625786</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2004 Jul 22;430(6998):471-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15269773</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2004 Aug;14(8):1594-602</Citation><ArticleIdList><ArticleId IdType="pubmed">15256511</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1827-31</Citation><ArticleIdList><ArticleId IdType="pubmed">1542678</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8449-54</Citation><ArticleIdList><ArticleId IdType="pubmed">8710891</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 1951 May;48(5):581-4</Citation><ArticleIdList><ArticleId IdType="pubmed">14838905</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 1962 Jun 11;55:953-9</Citation><ArticleIdList><ArticleId IdType="pubmed">13887466</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2004;55:41-68</Citation><ArticleIdList><ArticleId IdType="pubmed">15725056</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Genet. 2005 May;6(5):351-60</Citation><ArticleIdList><ArticleId IdType="pubmed">15861207</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Genet. 2005 Dec;1(6):e79</Citation><ArticleIdList><ArticleId IdType="pubmed">16389298</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Sep 15;313(5793):1596-604</Citation><ArticleIdList><ArticleId IdType="pubmed">16973872</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2006 Sep 22;126(6):1189-201</Citation><ArticleIdList><ArticleId IdType="pubmed">16949657</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Genet. 2006 Jun 2;2(6):e83</Citation><ArticleIdList><ArticleId IdType="pubmed">16741558</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Genet. 2006 Dec;38(12):1378-85</Citation><ArticleIdList><ArticleId IdType="pubmed">17072317</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Genet. 2007 Jan;39(1):61-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17128275</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Genet. 2007 Apr;39(4):457-66</Citation><ArticleIdList><ArticleId IdType="pubmed">17334365</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2007 Dec;177(4):1975-85</Citation><ArticleIdList><ArticleId IdType="pubmed">18073417</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2008 Jan;20(1):25-34</Citation><ArticleIdList><ArticleId IdType="pubmed">18239133</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2008 Mar 13;452(7184):215-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18278030</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Genet. 2008 Mar;4(3):e1000032</Citation><ArticleIdList><ArticleId IdType="pubmed">18369451</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2008 Apr 25;320(5875):489-92</Citation><ArticleIdList><ArticleId IdType="pubmed">18436779</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2008 May 2;133(3):523-36</Citation><ArticleIdList><ArticleId IdType="pubmed">18423832</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Genet. 2008 Jun;9(6):465-76</Citation><ArticleIdList><ArticleId IdType="pubmed">18463664</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2008 Jul;5(7):621-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18516045</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2008 Aug 7;454(7205):766-70</Citation><ArticleIdList><ArticleId IdType="pubmed">18600261</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2008 Dec;56(5):814-23</Citation><ArticleIdList><ArticleId IdType="pubmed">18665914</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods. 2009 Mar;47(3):142-50</Citation><ArticleIdList><ArticleId IdType="pubmed">18950712</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2009 Apr;12(2):133-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19179104</ArticleId></ArticleIdList></Reference><Reference><Citation>Epigenetics. 2009 Feb 16;4(2):119-24</Citation><ArticleIdList><ArticleId IdType="pubmed">19384058</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Freitag, Michael" sort="Freitag, Michael" uniqKey="Freitag M" first="Michael" last="Freitag">Michael Freitag</name><name sortKey="Mockler, Todd C" sort="Mockler, Todd C" uniqKey="Mockler T" first="Todd C" last="Mockler">Todd C. Mockler</name><name sortKey="Pellegrini, Matteo" sort="Pellegrini, Matteo" uniqKey="Pellegrini M" first="Matteo" last="Pellegrini">Matteo Pellegrini</name><name sortKey="Pomraning, Kyle R" sort="Pomraning, Kyle R" uniqKey="Pomraning K" first="Kyle R" last="Pomraning">Kyle R. Pomraning</name><name sortKey="Priest, Henry D" sort="Priest, Henry D" uniqKey="Priest H" first="Henry D" last="Priest">Henry D. Priest</name><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name><name sortKey="Wilhelm, Larry J" sort="Wilhelm, Larry J" uniqKey="Wilhelm L" first="Larry J" last="Wilhelm">Larry J. Wilhelm</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Vining, Kelly J" sort="Vining, Kelly J" uniqKey="Vining K" first="Kelly J" last="Vining">Kelly J. Vining</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002B21 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002B21 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:22251412
|texte= Dynamic DNA cytosine methylation in the Populus trichocarpa genome: tissue-level variation and relationship to gene expression.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22251412" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |